二代測序無提取FFPE直接建庫試劑盒操作流程
產(chǎn)品信息
1. TargetPlex NGS DNA Library Kit for Illumina
2. TargetPlex NGS DNA Library Kit for Ion Torrent
3. FFPE-Direct DNA Library Prep Kit for Illumina NGS
4. FFPE-Direct DNA Library Prep Kit for Ion Torrent NGS
5. HiFi PCR Master Mix For DNA library Amplification
6. Ultra HiFi PCR Master Mix for FFPE Direct Amplification
7. Illumina Library Amplification Primer Mix
8. 16S Metagenomics NGS Primer Pools
9. 16S Metagenomics NGS Primer Pools
10. 16S Metagenomics Library Kit
11. TargetPlex NGS NSCLC DNA Library Kit for Illumina
12. TargetPlex NSCLC NGS DNA Library Kit for Ion Torrent
13. TargetPlex NSCLC Primer Panel
14. 5X TargetPlex CHS Primer Pool
15. Illumina 16S NGS Library Kit (Two-Step PCR Method)
16. cfDNA NGS Library Kit for Illumina
17. cfDNA NGS Library Kit for Ion Torrent
18. CD Index Adaptors for Illumina NGS 1-8 Kit
19. CD Index Adaptors for Illumina NGS 1-16 Kit
20. CD Index Adaptors for Illumina NGS 1-96 Kit
21. Barcode Adaptors for Ion Torrent NGS 1-8 Kit
22. Barcode Adaptors for Ion Torrent NGS 1-16 Kit
23. Barcode Adaptors for Ion Torrent NGS 1-96 Kit
技術(shù)特點
· 無需脫蠟和預(yù)處理
· 單張FFPE可建庫
· 一管試劑制備（文庫）
· 超簡化的FFPE NGS工作流程
· 接頭兼容Ion torrent和Illumina平臺
NGS文庫制備試劑試劑優(yōu)勢
· 0.125-0.25張FFPE切片可完成建庫，切片樣本量要求小
· 免脫蠟，直提切片DNA
· 無需提取和純化DNA
· 手工操作時間短（10min）
· 步驟簡單，高效，成本低
· 靶向擴增和測序
· 也同時適用于新鮮組織樣本
· 可用于多種NGS測序平臺